姓名：张文豪
职称：副研究员
邮箱：whzhang@nankai.edu.cn
课题组网页：https://sky.nankai.edu.cn/sl/list.htm
研究方向：1、类全能性干细胞的开发及机制研究；2、多种单倍体细胞系的开发和应用。
教育背景与工作经历：
2025.03至今 南开大学药物化学生物学全国重点实验室 副研究员
2020.08-2025.03 南开大学药物化学生物学全国重点实验室 博士后
2017.09-2020.06 南开大学药学院 微生物与生化药学专业 理学博士
2013.09-2016.06 南开大学生命科学学院 遗传学专业 理学硕士
2009.09-2013.06 河南师范大学生命科学学院 生物技术专业 理学学士
研究成果：
近年来工作重点聚焦在解决单倍体干细胞的二倍化问题，开发各物种多类型单倍体细胞系，并在细胞命运调控和药物毒性靶点等方面全面开展覆盖全基因组的遗传学筛选研究，取得原创性系统性成果总结如下：
1.解析单倍体干细胞自发二倍化的关键机制；
2.利用单倍体系统高效揭示药物作用和细胞命运调控等方面的关键靶点基因。
代表性论文:
1.Zhang W#, Wang Q#, Sun S#, Liu Y#, Zhao Y, Luo H, Yao C, Sun Y, Ding D, Chang Y, Gao Q and Shuai L*. Derivation and genetic-screening of human haploid trophoblast stem cells. Science Bulletin (2025), S2095-9273(25)00175-6.
2.Zhang W#, Zhao Y#, Yang Z#, Yan J#, Wang H#, Nie S, Jia Q, Ding D, Tong C*, Zhang X*, Gao Q* and Shuai L*. Capture of totipotency in mouse embryonic stem cells in absence of Pdzk1. Advanced Science (2025), 12(6):e2408852.
3.Zhang W#, Sun S#, Wang Q#, Li X#, Xu M#, Li Q, Zhao Y, Peng K, Yao C, Wang Y, Chang Y, Liu Y, Wu X*, Gao Q* and Shuai L*. Haploid-genetic screening of trophectoderm specification identifies Dyrk1a as a repressor of totipotent-like status. Science Advances (2023), 9(51):eadi5683.
4.Zhang W#, Yao C#, Luo Y#, Li Q, Zhao Q, Zhao Y, Wang Y, Geng M, Wang Q, Xu M, Sun S, Wu D, Gao Q, Wu X*, Shuai L*. Rif1 and Hmgn3 regulate the conversion of murine trophoblast stem cells. Cell Reports (2022),38(13):110570.
5.Zhang W#, Tian Y#, Gao Q#, Li X, Li Y, Zhang J, Yao C, Wang Y, Wang H, Zhao Y, Zhang Q, Li L, Yu Y*, Fan Y*, Shuai L*. Inhibition of apoptosis reduces diploidization of haploid mouse embryonic stem cells during differentiation. Stem Cell Reports (2020), 15(1):185-197.
6.Zhang J#, Zhao Y, Tian Y, Geng M, Liu Y*, Zhang W*, Shuai L*. Genome-wide screening in the haploid system reveals Slc25a43 as a target gene of oxidative toxicity. Cell Death & Disease (2022), 13(3):284.
7.Sun S#, Zhao Q#, Zhao Y, Geng M, Wang Q, Gao Q, Zhang X*, Zhang W*, Shuai L*. BCL2 is a major regulator of haploidy maintenance in murine embryonic stem cells. Cell Proliferation (2023), 56(12):e13498.
8.Zhao Y#, Wang Q#, Zhang W#, Jia Q, Gao Q*, Shuai L*. Protocol to generate induced trophoblast stem cells from embryonic stem cells in mice. STAR Protocols (2023), 4(1):102092.
9.Xu M#, Zhao Y#, Zhang W#, Geng M, Liu Q, Gao Q*, Shuai L*. Genome-scale screening in a rat haploid system identifies Thop1 as a modulator of pluripotency exit. Cell Proliferation (2022), 55(4):e13209.
10.Gao Q#, Zhang W#, Zhao Y#, Tian Y, Wang Y, Zhang J, Geng M, Xu M, Yao C, Wang H, Li L*, Liu Y*, Shuai L*. High-throughput screening in postimplantation haploid epiblast stem cells reveals Hs3st3b1 as a modulator for reprogramming. Stem Cells Translational Medicine (2021), 10(5):743-755.
11.Xu M#, Zhang W#, Geng M#, Zhao Y, Sun S, Gao Q, Liu Y*, Shuai L*. Rapid generation of murine haploid-induced trophoblast stem cells via a Tet-on system. STAR Protocols (2021), 2(4):100881.
所获奖励：